Tissue engineering is the foothill of regenerative medicine owing to the development of artificial tissues and organs. This discipline involves engineering scaffolds that support cell growth and regeneration. Various materials, including polysaccharides derived from marine fauna, have been used to fabricate scaffolds. Seafaring animals, such as crustaceans, jellyfish, sponges, squids, and fish, have contributed significantly to the development of scaffold biomaterials as sources of polysaccharides. Most of them facilitate cell growth, adhesion, migration, and responses to molecular and physical environments. They have the advantages of low antigenicity, efficient extraction, and compatibility with other biomaterials, resulting in a biomimetic architecture. This chapter provides an overview of the recent advances in the development and application of marine animal polysaccharide-based scaffolds, focusing on their potential to treat various clinical conditions. This discussion also highlights emerging trends and future directions, emphasizing the role of sustainable marine biomaterials in advancing tissue engineering and regenerative medicine.