As a cornerstone of circular bioeconomy strategies, enzymatic hydrolysis transforms lignocellulosic biomass into fermentable monosaccharides (primarily glucose and xylose). This transformation is carried out using enzymatic systems (cocktails) that include cellulases, hemicellulases, and lytic polysaccharide monooxygenases (LPMOs). The derived sugars act as versatile precursors (platforms) for bio-based products, including fuels, plastics, and chemicals, enabling a transition from fossil resources to renewable alternatives. Nevertheless, several technical challenges limit the efficiency of enzymatic hydrolysis, as the inherent recalcitrance of the lignocellulosic biomass matrix. High enzyme costs, product inhibition, and mass transfer limitations, particularly at solids loadings above 15%, further restrict the process. Therefore, pretreatment methods are essential to alter biomass structure and improve enzyme accessibility. To overcome such limitations, optimization strategies target multiple fronts. Recent advancements in enzyme engineering include the development of stable, inhibition-resistant enzymes and optimized multienzyme cocktails, with LPMO incorporation proving particularly effective in addressing biomass accessibility challenges.