The conventional waste management systems in the Indian cities are constrained by rapid urbanization and population growth leading to generation of enormous volumes of waste. The approaches adopted to get rid of the waste are chemical-based, unsustainable, and damage the environment and public health. In the context of urban waste management, this chapter discusses the role of bio-enzymes as eco-friendly, cost-effective and scalable solution. The descriptive, qualitative case study-based chapter emphasizes the power of bio-enzymes in odor neutralization, bacterial control, and effective biodegradation especially in products which include microbe-driven action combined with natural cleaning agents. Grounded in qualitative analysis, the chapter investigates the role of policy frameworks and ecosystem enablers supporting integration of bio-innovation in urban sanitation striving toward Sustainable Development Goals. The qualitative study on 3 eco-friendly brands, Eco-365, Jalodbust and bluNgreen, emphasizes the necessity for inclusive policy frameworks to integrate municipal and environmental agendas, streamlined certification process to enable small enterprises access green compliance, skill development initiatives for sanitation workers for sustainable practices and public awareness campaigns to educate consumers on responsible consumption. The chapter, further, supports practical policy measures to popularize bio-enzyme innovations for a sustainable bio-future. It provides actionable insights for scaling green entrepreneurship in emerging urban ecosystems by positioning these efforts within the broader frameworks of smart cities.