Brassica species are gaining global attention for their health benefits, particularly due to bioactive compounds like glucosinolates and sulforaphane, which contribute to cancer prevention, neuroprotection, and metabolic health. However, commercializing Brassica-derived nutraceuticals and pharmaceuticals presents challenges in regulatory approval, sustainable cultivation, and technological advancements. Strict safety and efficacy assessments by regulatory bodies such as the FDA and EFSA are essential to ensure scientific validation of health claims and determine safe consumption levels. Sustainable farming methods, including hydroponics, aeroponics, and vertical farming, are improving bioactive compound accumulation while minimizing environmental impact. These approaches optimize growth conditions and enhance phytochemical composition, making Brassica crops more suitable for functional food applications. Additionally, artificial intelligence (AI) and machine learning (ML) are transforming Brassica research by accelerating genomic selection for high-yielding, nutrient-rich varieties. AI-driven precision agriculture further refines cultivation techniques, maximizing phytochemical content and improving efficiency in pharmaceutical development. This chapter explores the commercialization of Brassica-based nutraceuticals and pharmaceuticals, emphasizing the importance of regulatory frameworks, sustainable farming practices, and innovative technologies in unlocking their full potential.