Globally, mustard is considered to be the most significant vegetable oil source. Due to their great susceptibility to biotic stresses, brassica crops substantially impact crop quality and yield. Although different pathogens quickly overcame that resistance by altering their metabolic pathways, breeding approaches were previously employed to build resistance in mustard to minimize biotic stresses. It is imperative to use cutting-edge methods to create biotic stress-tolerant plants to meet the challenge of comprehending the physiological pathways of both pathogens and plants. Various measures are necessary to prevent stress, including selecting genes, metabolites, enzymes, and microbes to survive it. Several strategies, including using DNA markers in breeding, identifying quantitative trait loci, tissue culture, etc., are practical methods to increase mustard’s resistance to biotic stress. These methods must be combined to create high-yield, healthy cultivars. Methods such as in vitro tissue culture and identifying quantitative trait loci (QTL) may be more beneficial to increasing mustard’s resistance to biotic stress. These methods must be combined to create high-yield, healthy cultivars.