Vegetables are a vital source of essential nutrients and play a crucial role in enhancing human health. The Brassicaceae family, also known as crucifer, includes various crops such as cabbage, mustard, turnip, and radish, which provide a sufficient amount of nutrition (vitamins, minerals, and antioxidants). Despite their nutritional benefit, malnutrition and micronutrient deficiency remain serious global issues, particularly in developing countries. To combat this serious concern, biofortification has proven to be a suitable solution. Various techniques like agronomical practices, breeding, and transgenic approaches have been applied to crops to increase their nutrient content. Agronomical biofortification involves the application of nutrients at the soil and foliar levels, seed priming, and soilless cultivation. Conventional breeding enhances genetic characters for nutritional improvement. Different transgenic methods like CRISPR/Cas9 and genome editing further increase the nutritional content of crops. The integration of these technologies can be used to improve human health and ensure the global availability of nutritious food.