This paper reports the development of an alkaline passive direct methanol fuel cell (DMFC) and its activation process to achieve the optimal cell performance. The fuel cell’s active area measures 25 cm2 and uses a commercial Fumasep membrane with a thickness of 130 μm. The activation protocol includes a constant voltage test for 10 min, an Open circuit voltage (OCV) test for 30 min, followed by the polarization test. The cell performance increases continuously with the activation cycles and reaches an optimum. In this study, we have found the optimum performance at the seventh activation cycle. The result shows a reduction in the activation loss and ohmic loss during the activation process, which indicates the improvement in the electrochemical reaction and the ion conductivity of the membrane. This is the ultimate objective of the activation process.