Fluorescein stain can be used efficiently and cost effective way for examining the raw ophthalmic specimens visualizing tissue details under microscopic examinations. Author(s) developed this staining method in various raw ophthalmic specimens to detect micro-organisms including parasites, corneal and conjunctival pathologies, lid, orbital pathologies and in enucleated eyeballs. The staining process is very simple and can be performed quickly spending minimum time. The staining also gave important informations about the retinal pigment epithelium, retina, intraocular cancer seedings etc. The novelty of the stain had wider applications in detecting fungus, viral inclusion bodies, parasites, immunological and tumor cells covering anatomy, pathology, onco-pathology, microbiology, parasitology, virology, immunology, entomology and so on.