Harmful agriculture practices like overuse of chemical fertilizers have led to a deteriorating environment. Hence it is a necessity to discover alternative eco-friendly methods to overcome this issue. Phosphorus is an essential nutrient required for the growth and development of plants. The deficiency of phosphorus in plants inhibits shoot growth, leading to necrosis or hampering growth. The supply of phosphorus is mainly fulfilled through the use of chemical fertilizers which causes bio magnification of chemical compounds in plants, thus affecting our health ultimately. Therefore, an eco-friendly alternate to fulfill phosphorus requirement is imperative. Algae, being rich in its biochemical composition, has the ability to biologically accumulate metals thereby acting as promising avenue for nanoparticle synthesis. The present paper highlights the method of algae-based phosphorous nanoparticles (ABPNP) development using a green microalga and its incorporation in soil as biofertilizer using model plant species Vigna radiata. The results indicated the enhanced growth rate of V. radiata in terms of leaves, branching and height. The findings offer insights into the development of eco-friendly biofertilizers for sustainable agricultural practices.