Chapter 4 explores the development and maturation of the modern Chinese university spirit during the period from 1924 to 1937. It highlights the rise of universities such as Tsinghua, Nankai, and those in the Nanjing district (including Shanghai), which diversified the representation of university spirit beyond Peking University. The chapter discusses how the university spirit evolved into a relatively mature and stable phase, with distinctive characteristics emerging across various institutions. It also examines the influence of missionary universities and their Christian spirit, which played a role in shaping the educational landscape despite their controversial impact on China’s cultural traditions. The blending of Chinese and Western university spirits is emphasized, showcasing how modern Chinese universities integrated traditional Chinese values with Western achievements to foster innovation. The chapter provides an overview of the university spirit’s manifestations, evolution, and specific case studies to analyze its historical development.