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Tomato leaf curl New Delhi virus (ToLCNDV) is one of the most devastating viruses affecting our tomato crops. Besides the tomato crops, this virus has also been seen to infect other crops of the Solanaceae family and other similar families like Fabaceae and Malvaceae. Many traditional methods, along with the use of heavy pesticides, seem to be ineffective in combating this particular virus. In this book chapter, CRISPR/Cas9 is being used as a powerful technology against this deadly virus. CRISPR/Cas9 has evolved as a robust genome editing tool, following zinc finger nucleases and transcription activator-like effector nucleases. The mechanism of CRISPR/Cas9 follows three basic steps, which include target search and recognition, target cleavage, and the repair of double-strand breaks (homology-directed repair and nonhomologous end joining). This book chapter highlights the effective use of CRISPR/Cas9 against the Tomato leaf curl New Delhi virus. The last section of this chapter also includes the limitations of the system along with the strategies used in overcoming them. Besides this, the use of prime editing and Cas9 nuclease as an advanced alternative to CRISPR/Cas9 is also being discussed.

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Employing CRISPR-Cas9 Genome Editing Tool Against Tomato Leaf Curl New Delhi Virus: Prospects and Retrospect

  • 章节

  • 发表日期：

    2026年

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  • Sanskriti Shiwani,
  • Mobassareen,
  • Auroshikha Mohanty,
  • Ravi Ranjan Kumar,
  • Mohammad Ansar,
  • Kumari Shashi Bala,
  • Chandan Kishore,
  • Tushar Ranjan

摘要

Tomato leaf curl New Delhi virus (ToLCNDV) is one of the most devastating viruses affecting our tomato crops. Besides the tomato crops, this virus has also been seen to infect other crops of the Solanaceae family and other similar families like Fabaceae and Malvaceae. Many traditional methods, along with the use of heavy pesticides, seem to be ineffective in combating this particular virus. In this book chapter, CRISPR/Cas9 is being used as a powerful technology against this deadly virus. CRISPR/Cas9 has evolved as a robust genome editing tool, following zinc finger nucleases and transcription activator-like effector nucleases. The mechanism of CRISPR/Cas9 follows three basic steps, which include target search and recognition, target cleavage, and the repair of double-strand breaks (homology-directed repair and nonhomologous end joining). This book chapter highlights the effective use of CRISPR/Cas9 against the Tomato leaf curl New Delhi virus. The last section of this chapter also includes the limitations of the system along with the strategies used in overcoming them. Besides this, the use of prime editing and Cas9 nuclease as an advanced alternative to CRISPR/Cas9 is also being discussed.