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In order to synthesize uniform, evenly distributed silver nanoparticles (AgNPs), a straightforward chemical reduction technique was used. In this work we synthesized the particles by using ascorbic acid as a surfactant and trisodium citrate as reducing agent to reduce AgNO3 to metallic silver. UV–visible spectroscopy was the primary method used to confirm the formation of AgNPs. At 422.84 nm, it displays the surface plasmon resonance. The nanoparticles were characterised by FTIR, XRD, DLS, Zeta potential, FESEM and EDAX. HR TEM and SAED are taken to measure the size and crystalline nature of the nanoparticles. It demonstrates that the produced AgNPs have an average diameter of 30 nm and were quasi spherical in shape and evenly distributed. The anticancer and cytotoxic properties of the samples were examined. MTT assay performed in mouse fibroblast L929 cells have substantiated the cytocompatibility of the silver nanoparticles in normal cells. Significant cytotoxicity was observed in SKOV-3 ovarian cancer cells. The anti-cancer potential of the silver nanoparticles was validated through AO/EtBr double staining technique which shows the apoptotic potential of the nanoparticles. Annexin V apoptotic assay established the percentage of apoptotic cells in nanoparticle treated group compared to that in control group. The results highlight the anti-microbial and anti-cancer efficacy of chemically synthesized AgNPs.

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Investigating the Anti-cancer Potential of Silver Nanoparticles Synthesized by Chemical Reduction of AgNO3 Using Trisodium Citrate and Ascorbic Acid

  • 会议论文

  • 发表日期：

    2025年

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  • K. S. Dhanya,
  • M. Anaswara Biju,
  • U. Arun,
  • R. Sreeja

摘要

In order to synthesize uniform, evenly distributed silver nanoparticles (AgNPs), a straightforward chemical reduction technique was used. In this work we synthesized the particles by using ascorbic acid as a surfactant and trisodium citrate as reducing agent to reduce AgNO3 to metallic silver. UV–visible spectroscopy was the primary method used to confirm the formation of AgNPs. At 422.84 nm, it displays the surface plasmon resonance. The nanoparticles were characterised by FTIR, XRD, DLS, Zeta potential, FESEM and EDAX. HR TEM and SAED are taken to measure the size and crystalline nature of the nanoparticles. It demonstrates that the produced AgNPs have an average diameter of 30 nm and were quasi spherical in shape and evenly distributed. The anticancer and cytotoxic properties of the samples were examined. MTT assay performed in mouse fibroblast L929 cells have substantiated the cytocompatibility of the silver nanoparticles in normal cells. Significant cytotoxicity was observed in SKOV-3 ovarian cancer cells. The anti-cancer potential of the silver nanoparticles was validated through AO/EtBr double staining technique which shows the apoptotic potential of the nanoparticles. Annexin V apoptotic assay established the percentage of apoptotic cells in nanoparticle treated group compared to that in control group. The results highlight the anti-microbial and anti-cancer efficacy of chemically synthesized AgNPs.