Veterinary mycology is an important field of veterinary science that focuses on the identification, diagnosis, and treatment of infectious and zoonotic fungal diseases that pose risks to both animal and public health. The emergence of new and resistant fungal pathogens is influenced by human-animal-environmental interactions. In Southeast Asia, the environmental conditions with warm, humid climate, dense biodiversity, urbanization, and habitat disruption can influence the growth and transmission of these novel fungal pathogens. This chapter highlights the importance of fungal pathogens, zoonotic transmission, and the impact of environmental factors and climate change in the transmission and evolution of fungal diseases. It describes the growing concerns and challenges of antifungal resistance and the increasing cases of fungal diseases, especially those with zoonotic potential. In response to these challenges, this chapter reviews recent and current applications of advanced diagnostic techniques used for the timely identification of fungal pathogens, improving the accuracy and timeliness of disease reporting, treatment, and control. Development of antifungal drugs is also addressed in this chapter with more emphasis on the importance of a collaborative response, that is, a One Health approach involving veterinary, medical, and environmental health to foster more effective surveillance systems and intervention strategies. Moreover, this chapter explores the future outlook of interdisciplinary collaboration when it comes to fungal disease monitoring, antifungal drug development, and sustainable research funding to mitigate the growing threat of fungal diseases and strengthen animal, human, and environmental health.