The rising population causing a significant threat to meeting the world food demand results in increased use of chemical fertilizers. Excessive use of chemical fertilizers causes challenges like soil degradation, fertility loss, mineral deficiency, pest resistance, climate change, and chemical pollution. These emerging issues impose a considerable burden on existing agricultural problems. Biostimulants are a good initiative to overcome agricultural issues by improving overall production, sustainability, metabolic reactions, and physiochemical response of plants against various biotic and abiotic stresses. Seaweeds, plant extracts, hydrolyzed proteins, nitrogen-containing compounds, humic substances, and microorganisms are various biostimulants. Microbial biostimulants are helpful to lower the use of chemical fertilizers, but their efficacy is greater when combined with nanostimulants. Nanotechnology is an advanced field that creates nano-sized particles and is used in every field of life. In agriculture, nanoparticles improve crop production and remove agro-waste from water bodies. Nanoparticles (both organic and inorganic) are synthesized by biological, chemical, and mechanical processes. Green synthesized nanoparticles (NPs) are considered environmentally friendly as compared to mechanical and chemical approaches due to less production of toxic chemicals. Nanomaterial integration with microbial biostimulants into agricultural practices boosts crop productivity. Integrating microbial nanotechnology into agricultural practices and biomedicine is promising, but it must overcome synthesis, standardization, and scalability challenges.