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Giant unilamellar vesicles (GUVs), which mimic cell membranes, are primarily used as exteriors of cell-type molecular robots. A method for producing GUVs that are uniform in size and structure and have high encapsulation efficiency is required to ensure the designed performance of robots. In this section, we describe a system for producing GUVs using microfluidic devices. This system, utilizing the interfacial transfer of water-in-oil (W/O) droplets, can produce monodisperse GUVs with diameters of 25–45 µm with a transfer yield of ~ 100%. To apply this system to constructing molecular robotics, we also present the results of encapsulating an in vitro transcription/translation system in GUVs and examining the conditions under which protein synthesis and reconstitution of membrane proteins are processed.

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Preparation of Monodisperse GUVs Using a Microfluidic Device

  • 章节

  • 发表日期：

    2026年

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  • Mamiko Tsugane,
  • Hiroaki Suzuki

摘要

Giant unilamellar vesicles (GUVs), which mimic cell membranes, are primarily used as exteriors of cell-type molecular robots. A method for producing GUVs that are uniform in size and structure and have high encapsulation efficiency is required to ensure the designed performance of robots. In this section, we describe a system for producing GUVs using microfluidic devices. This system, utilizing the interfacial transfer of water-in-oil (W/O) droplets, can produce monodisperse GUVs with diameters of 25–45 µm with a transfer yield of ~ 100%. To apply this system to constructing molecular robotics, we also present the results of encapsulating an in vitro transcription/translation system in GUVs and examining the conditions under which protein synthesis and reconstitution of membrane proteins are processed.