This study aims to transform waste into wealth by adapting agro-industrial waste, specifically spent tea, as an insoluble carrier support for laccase immobilization. The porous structure, low cost, biodegradability, and ease of functionalization of spent tea make it a promising solution to address the issues with free laccase, such as low stability and rapid activity loss when applied for industrial use. Successful acid-base modification of spent tea was observed in this study using FTIR analysis where two new peaks (1631 and 1541 cm−1) were appeared and stretching of carboxylic acid group at 3500–300 cm−1. The study also investigates laccase immobilization on modified spent tea, manipulating the incubation period as a variable. It is revealed that the highest activity recovery of 35.97% was at 2 hr incubation period. Not only that, the FTIR analysis also confirmed the successful immobilization, with the presence of N-O-H functional groups. To reach the research objective of developing an economical and sustainable approach for using laccase in the industry a reusability study demonstrates the immobilized laccase’s sustained enzymatic capability over five cycles. This highlights the stability of the immobilized laccase, making it a cost-effective solution for industrial applications.