With the development of science and technology and the popularization of the concept of green travel, cycling has become a popular way of travel, but traditional cycling navigation devices (such as mobile phones and car GPS) have obvious defects. This paper studies the user experience and acceptance of cyclists on the intelligent navigation system based on VR glasses, uses VR technology to optimize cycling navigation, and conducts data analysis on the actual experience of users in using the intelligent navigation system. This paper analyzes user experience data through experiments. The experimental results show that in mountain biking scenarios, the system’s 3D terrain modeling and obstacle warning functions significantly improve user satisfaction, which is 40% higher than traditional navigation. The system has been highly recognized by cyclists in terms of improving navigation convenience and immersion, but it still needs to be optimized in terms of wearing comfort and rationality of information display. This paper provides a valuable reference for the innovation of cycling navigation technology, and helps promote cycling navigation to a more humane and efficient direction.