The study investigates the effects of bacterial impregnation and mineral admixtures, fly ash, and ground granulated blast furnace slag (GGBS), on the mechanical and durability properties of concrete. Various concrete mixes, including normal concrete (NC), bacterial concrete (BC), fly ash concrete (FC), GGBS concrete (GC), bacterial fly ash concrete (BFC), and bacterial GGBS concrete (BGC), were evaluated for compressive strength, split tensile strength, water absorption, and porosity. The results indicate that bacterial concrete enhances compressive strength, with the most significant improvement observed in BGC at later curing ages. Split tensile strength also increased, with BGC exhibiting the highest values, demonstrating the combined benefits of bacterial calcite precipitation and GGBS densification. Durability assessments showed that bacterial impregnation significantly reduced water absorption and porosity, with BGC achieving the lowest values, indicating a denser and more impermeable matrix. These findings suggest that bacterial concrete, particularly with GGBS, can serve as a viable alternative for enhancing both strength and durability in sustainable construction. The study highlights the potential of bacterial concrete for developing eco-friendly, high-performance structures with improved long-term durability.