Bamboo, a sustainable and eco-friendly material, has a collection of significant attention in the construction industry because of its rapid growth, excellent strength-to-weight ratio, and widespread availability in tropical and subtropical regions. As a natural composite material, bamboo exhibits excellent mechanical properties, making it a promising alternative to traditional steel reinforcement in concrete structures. Current study explores the potential of bamboo as reinforcing material in concrete columns, aiming to offer a sustainable, cost-efficient, and eco-friendly solution for modern construction practices. The experimental investigation involves the preparation and testing of bamboo-reinforced concrete columns to evaluate their structural behaviors under compressive loads. Standardized tests are conducted to measure the strength in compression, failure modes, and overall behavior of the reinforced columns. The bamboo is treated to enhance its durability and bonding with concrete, ensuring it can effectively replace or supplement steel reinforcement. The study includes a comparative analysis between different bamboo knot spacing to assess the viability of bamboo as an alternative reinforcement material. The findings of this research will indicate that the behaviors of different knot spacings of bamboo-reinforced columns under compressive loads and also comparing the theoretical loads and experimental loads under compression. Additionally, the research aims to examine the compressive strength of bamboo-reinforced concrete columns and identify factors influencing their performance. By analyzing the structural integrity and failure patterns, the study seeks to establish guidelines for the practical application of bamboo reinforcement in concrete construction. The findings will contribute to the development of sustainable construction materials, promoting the use of renewable resources in the building industry.