Settlement of migrants including refugees in regional areas is a complex task involving various socio-economic aspects. There is a range of business support available in regional Australia, provided by both government and non-government organisations. This support can help businesses of all sizes, for those starting from the beginning to established businesses, to grow and succeed. Over 15% of all businesses supported reside in regional Australia. This number is bound to grow due to the government commitment to increase the number of migrants including refugee intake to regional Australia. Migrants and refugees have a great desire to start a business in Australia. Thrive Refugee Enterprise is a not-for-profit organisation dedicated to assisting refugees and asylum seekers potential entrepreneurs to become financially independent by starting or growing their own small businesses. Thrive Refugee Enterprise is proud to support over 800 businesses that have started due to tailored support for the unique needs of refugees and asylum seekers and providing them with the knowledge of how the Australian business environment operates. This chapter is dedicated to discussing the refugees and asylum seekers economic participation in regional Australia through business entrepreneurship and highlighting the challenges facing the refugees to participate economically.