In the current higher education system, “innovation and entrepreneurship” has become the core of university education reform. In this context, the talent training model of university is gradually transforming to cultivate professionals with innovative spirit and entrepreneurial ability. As a key place for cultivating students’ practical skills and innovative literacy, the university lab plays a vital role in the education system and shoulder the important task of implementing experimental teaching and practical operation. However, the existing university lab faces challenges in the operation process, including but not limited to insufficient security protection, complex equipment management, scattered teaching resources, and inaccurate data statistics. In view of this, based on artificial intelligence and Internet of Things (IoT) technology, we propose a set of innovative solutions for the construction of a smart lab in the university adopting distributed collaborative operation and centralized management. The solution aims to realize the intelligent safety monitoring of the experimental environment, the comprehensive management of the experimental process, and the effective integration and sharing of experimental data by building an IoT control system and a smart lab integrated management platform.