The aim of this study was to examine the scan patterns and heart rate variability during simulated flight tasks. Twelve college students participated in flight missions simulated on a simulator. Each cadet's scan patterns and heart rate variability were recorded using a commercial eye tracker and a small ECG recording device strapped around the chest, respectively. The areas of interest (AOIs), pupil diameters, fixation numbers, average fixation time, and heart rate variability were then analyzed and compared across various phases: take-off and initial climb, turning and climbing, cruising on the downwind leg, turning and descending, final approach, and landing. Our analysis revealed significant variations in scan patterns and heart rate variability during different phases of simulated flight. Specifically, the final approach and landing, turning and descending, and take-off and initial climb phases exhibited more mental workload compared to other phases.