In this chapter, the notion of infrastructures for research is developed based on an analysis of the Framingham Heart Study in the United States. This study was established for research on the causes of cardiovascular disease in the late 1940s and continues to this day. For this longitudinal study, the concept of infrastructure can help make sense of both the way certain forms of research are facilitated or restricted by its organizational and material set-up and of how the study is imagined and projected as a landmark in modern science. On the one hand, initial choices of, for example, methods and hypotheses had a lasting influence on the study, even as new (e.g., genomic) approaches to medical research emerged. On the other hand, the study’s presumed basis in local community and family relations is projected as one of its continuing strengths. As a result, The Framingham Heart Study’s continued relevance for and contributions to medical research remain rooted in its initial emphasis on individual behaviours as the most important risk factors for cardiovascular disease across the multiple layers that presently constitute the infrastructure.