In this study, nutrient uptake efficiency of non-legume species in green manuring field for sustainable environment was examined to understand their potential for improving soil nutrient quality. The non-leguminous species assessed in this study included oil radish, forage radish, February orchid, and rapeseed with ryegrass serving as the control. The findings showed notable improvements in nutrient uptake, especially for nitrogen (N), phosphorus (P), and potassium (K). Although, in the Hunan province, Forage radish achieved the highest N and P uptake, with increases of 322 and 101%, while in Sichuan it was 277 and 469% respectively, compared to ryegrass. The greatest potassium accumulation was observed in forage radish (123%) and February orchid (243%). Phosphorus use efficiency was also notably high in forage radish, with increases of 301% in Hunan and 633% in Sichuan. Additionally, soil enzyme activity levels such as β-glucosidase and leucine-aminopeptidase were enhanced by up to 367% with oil radish, while phosphatase and N-acetyl-glucosaminidase activities were highest in forage radish. These findings suggest that non-legume green manures, particularly forage radish and oil radish, can improve soil nutrient availability and enhance enzyme activity, making them suitable for sustainable nutrient management practices.