The production of biodiesel generates significant quantities of waste glycerol. It is a byproduct posing significant environmental challenges. Often disposed of improperly, it can contaminate soil and water sources, contributing to pollution and ecological harm. Utilization of this unused toxic waste glycerol for the production of environment-friendly biomaterial would be highly beneficial. Furthermore, one-time-use plastics persist for decades, breaking down into harmful microplastics that threaten ecosystems worldwide. Bioplastics offer a promising alternative, but their widespread adoption is hindered by high production costs, reliant on costly carbon substrates. This study addresses this dual challenge by repurposing waste glycerol as a cost-effective substrate for bioplastic production. Halobacterium noricense, sourced from rock salt, was cultivated for the synthesis of polyhydroxyalkanoate (PHA) using waste glycerol as a sustainable carbon substrate. Extraction of PHA was achieved through hydrolysis, followed by purification and precipitation using chloroform and methanol, respectively. The findings reveal promising results, with polyhydroxyalkanoate yields of 0.8 g/L, 1.62 g/L, and 3.72 g/L achieved from waste glycerol concentrations of 1%, 2%, and 5%, respectively. This innovative approach offers a sustainable and economically viable alternative to traditional plastics, contributing to environmental remediation and resource efficiency.