Integrating technology into classroom instruction, particularly in biology, can significantly enhance student learning outcomes. This study aims to develop a contextualized interactive e-module through the Kotobee application as a learning tool for digital-based microlearning strategy in biology, focusing on Grade 10 students’ least mastered competencies. It has utilized a descriptive-developmental approach combined with one-group pretest-posttest quasi-experimental research design to determine the usability of the developed material. Validation ratings showed that the interactive e-module is highly usable and engaging, with interactive features that encouraged mastery of key concepts. Results on the material utilization revealed a significant improvement in students’ pre-test and post-test scores, indicating usability that can aid comprehension and retention of complex biology topics. These suggest that the material is a valuable learning tool for supporting microlearning, promoting active learning, and improving academic outcomes. The study recommends widespread adoption of this interactive e-module in biology instruction and proposes educator training workshops to optimize its use.