GABA, a crucial signalling molecule, influences stress tolerance, growth, and metabolic regulation in plants. Advances in genetic engineering and genome editing techniques, particularly clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR–Cas9), have facilitated precise modifications in GABA biosynthesis and signalling pathways, offering new avenues for crop improvement. GABA biosynthesis pathways in plants engage three enzymes viz., Glutamate decarboxylase (GAD), GABA transaminase (GABA-T), and succinic semialdehyde dehydrogenase (SSADH). Engineering of these pathways via gene overexpression, silencing, or targeted genome editing promotes stress resilience and plant productivity. This advocates for a discussion on the current strategies for genetic manipulation and recent advancements in CRISPR-based approaches for fine-tuning GABA pathways, mitigating stress tolerance, and augmenting crop performance. While genetic modifications of GABA metabolism present promising opportunities, challenges such as regulatory hurdles and potential off-target effects must be addressed. Future research integrating multi-omics approaches and synthetic biology tools would help in accomplishing efficacious GABA bioengineering and the development of climate-resilient crops, ensuring sustainable agricultural productivity and food security.