The textile industry faces significant challenges. The integration of enzymes offers a promising solution as various enzymes, including proteases, cellulases, and amylases, facilitate processes such as bio-polishing, de-sizing, and bleaching, thereby reducing reliance on harsh chemicals and labor-intensive methods. The use of enzymes not only improves fabric quality but also minimizes water and energy consumption while reducing waste generation. Specific enzymes serve distinct functions in textile processing. Cellulase improves the softness and absorbency of cotton fabrics, while amylase effectively removes starch-based sizing agents without fiber damage. Catalases break down residual hydrogen peroxide after bleaching, ensuring cleaner dyeing processes without extensive purification steps. Despite these benefits, challenges remain in scaling up enzyme applications for industrial use in the textile sector. Current limitations include integration into existing manufacturing infrastructure and cost-effective enzyme production. Ongoing research addresses these challenges through computational analysis of enzymatic behaviors and advances in microbial biotechnology. This chapter examines how microbial enzymes are redefining textile bioprocessing, aligning with sustainability goals and improving production methods. The development of more stable and specific enzymes promises to establish better practices in the textile industry while minimizing environmental damage.