The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system is a powerful tool for modifying crop genome that has recently attracted much attention due to the ability to modify crops’ genomes with high precision and specificity. This system based on bacterial immune mechanisms enables meaningful editing of genomes of crop plants, which opens up new possibilities to increase yield, tolerance to stress and diseases. Additional to RNA interference (RNAi) to suppress unwanted target genes, CRISPR-Cas has significantly extended the field of molecular breeding and biotechnology. Instead, new solutions have been developed that expand the use of CRISPR: base and prime editing; these do not work with double-strand breaks, so there is no need for homologous recombination and less likelihood of escaping the site of action to harm a new location. Previously, Cas systems are used for DNA targeting only while now for RNA targeting Cas12 and Cas13 are used for multiple used in pathogen defense. Furthermore, gene drives and epigenetic approaches using CRISPR open up new directions in work on stable durable even transgenerational crop improvement. In RNA interference process, small interfering RNAs (siRNAs) and microRNAs (miRNAs) have been used for maintaining gene expression controlling biotic and abiotic stresses. The combination of CRISPR and RNAi thus enhances multiple features of interest including silencing of genes responsible for susceptibility to diseases, and at the same time amplifying positive attributes including nutritional value and efficiency of growth. Use of CRISPR-Cas technology in crop improvement has broad implications for dealing with global issues as those of food shortage as well as climate change. Efficient if stacking systems are available through multiplexed editing, high-throughput screens and synthetic biology interfaces CRISPR, which is important for next-generation crops. As regulatory approaches change, it is apparent that CRISPR-edited crops will be a key part of feeding the world’s population further as well as meeting the challenge of maintaining agricultural production in the light of climate variability.