Honey bees play a crucial role in ecosystems, offering essential ecosystem services, such as provisioning, regulating, and cultural services. The application of nanotechnology is expanding in various fields, including agriculture and apiculture. While the use of nanomaterials in these sectors holds promise, it can have negative consequences for honey bees, potentially affecting their pollination efficiency. These bees are exposed to nanomaterials through various routes, viz., application to crops, beekeeping activities, and environmental deposition. Such exposure can affect their biology, physiology, survival, and behaviour. This chapter explores the potential effects of nanomaterials on honey bees, drawing on relevant studies.