The area of tissue engineering is constantly changing due to multidisciplinary research employing cutting-edge technologies from engineering, molecular biology, synthetic chemistry, pharmaceutics, and medicine, among other disciplines. Tissue engineering is now fast moving toward therapeutic applications and has advanced beyond in vitro and animal research as a result. Tissue engineering technology advances toward nanoscale material creation techniques as it develops. Therefore, tissue engineers want adaptable imaging techniques that can track not only morphological but also functional and molecular information to evaluate improved tissue engineering applications. Many tissue engineering investigations proceed with utilizing the use of traditional instruments, such as histology procedures, which offer valuable but constrained information, particularly when it comes to in vivo preclinical and clinical approaches [1]. Because the samples must be destroyed in order to visualize tissue-engineered constructions using these traditional techniques, longitudinal three-dimensional (3D) volumetric measurement is severely constrained. Additionally, histology needs statistical analysis to account for discrepancies in experimental results from different samples and at different times due to destructive methods and limited views within the confined volume. Tissue engineers have access to a wide range of sophisticated imaging methods, and more and more current tissue engineering research efforts are being conducted to investigate the uses of several sophisticated imaging modalities [2–4]. The limitations of traditional instruments can be overcome by using advanced imaging techniques to monitor tissue-engineered constructions noninvasively, longitudinally, and consistently.