Toxicity tests are essential to provide information regarding an emerging substance’s harmfulness to health and the environment. Nevertheless, toxicological studies do not keep pace with the development and production of emerging drugs or materials regarding their potential toxic effects. In addition, international toxicological controls rely mainly on vertebrates, which is not in alignment with a world committed to implementing the 3Rs (replacement, reduction, and refinement). The zebrafish has become a popular animal model for toxicity research studies due to its numerous natural advantages (i.e., prolific breeding, rapid development, and small size), making it a valuable tool for performing short-term, space-saving, and inexpensive screenings.