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Protease as a protein hydrolyzer is an imported enzyme in Indonesia. To produce local enzyme, indigenous bacteria can be used as an alternative source of enzyme. This study aims to determine protease stability of indigenous Lactobacillus fermentum EN-17-2 and Lactobacillus plantarum B110 at time of storage. Storage times was carried out for 0, 7, 14, 21 and 28 days. The protease activity was detected using tyrosine method. Relative activity was stated stable with a value of ≥ 50%. Data were analyzed statistically using ANOVA. The results showed that the protease stability of L. fermentum EN17-2 at frozen temperature for 28 days with a relative activity of 60.06% was not significantly different from 0 day, on the contrary, at cold temperatures the protease stability showed a significant difference (p < 0.05). The protease stability of L. plantarum B110 for 28 days at cold temperature with a relative activity of 52.26%, and at freezing temperature with a relative activity of 50.51% was significantly different from 0 day (p < 0.05). Based on the stability of protease, the protease stability of L. fermentum EN17-2 was better than that of L. plantarum B110. It is recommended to use L. fermentum EN17-2 as a source of protease-producing lactic acid bacteria.

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Protease Stabilities of Lactobacillus fermentum EN17-2 and Lactobacillus plantarum B110 Indigenous at Various Storage Temperatures and Times

  • 会议论文

  • 发表日期：

    2025年

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  • T. Khusniati,
  • M. Fajar,
  • Y. S. Juariah,
  • Sulistiani

摘要

Protease as a protein hydrolyzer is an imported enzyme in Indonesia. To produce local enzyme, indigenous bacteria can be used as an alternative source of enzyme. This study aims to determine protease stability of indigenous Lactobacillus fermentum EN-17-2 and Lactobacillus plantarum B110 at time of storage. Storage times was carried out for 0, 7, 14, 21 and 28 days. The protease activity was detected using tyrosine method. Relative activity was stated stable with a value of ≥ 50%. Data were analyzed statistically using ANOVA. The results showed that the protease stability of L. fermentum EN17-2 at frozen temperature for 28 days with a relative activity of 60.06% was not significantly different from 0 day, on the contrary, at cold temperatures the protease stability showed a significant difference (p < 0.05). The protease stability of L. plantarum B110 for 28 days at cold temperature with a relative activity of 52.26%, and at freezing temperature with a relative activity of 50.51% was significantly different from 0 day (p < 0.05). Based on the stability of protease, the protease stability of L. fermentum EN17-2 was better than that of L. plantarum B110. It is recommended to use L. fermentum EN17-2 as a source of protease-producing lactic acid bacteria.