Functional bacteria are a group of soil bacteria that serve as suppliers of Nitrogen (N) and Phosphorus (P) nutrients, growth promoters, and pathogens control. Furthermore, these bacteria can be inoculated into the seeds as biopriming to accelerate germination and optimize the potential of the seed. Therefore, this experiment aims to obtain and determine the effectiveness and activity of 5 functional bacterial as biopriming agents in accelerating the germination of 4 types of seeds in vitro. In addition, the Completely Randomized Design was used with 5 identified functional bacteria, namely Bacillus siamensis InaCC B1435 (A), Chromobacterium alkanivorans InaCC B1433 (B), Chromobacterium alkanivorans InaCCB1436 (C), Stenotrophomonas maltophilia InaCCB1434 (D), Chromobacterium violaceum InaCC B1437 (E), and 4 types of seeds namely Ipomoea reptana Poir, Leucaena leucocephala L., Sorghum bicolor L., and Cucumis sativus L. All treatments and control were conducted in triplicate. The result showed that the functional bacteria activity in inoculants A, B, C, D, and E can accelerate the seed germination of I. reptana Poir and C. sativus L., bacteria in inoculants B and D can accelerate the seed germination S. bicolor L., and inoculants B (Chromobacterium alkanivorans InaCCB1433) was able to accelerate the seed germination of all tested seeds (I. reptana Poir, L. leucocephala L., C. sativus L L., and S. bicolor) with the highest yield on all variables of bacterial activity as a biopriming agent, namely germination percentage (80, 50, 70, and 20%), sprouts length (9.64, 4.14, 8.89, and 9.06 cm), and vigor index (771.2, 207, 711.2, and 181.2).