In the manufacture of composites, synthetic adhesives are generally used, which cause formaldehyde emissions and impact human health and the environment. Therefore, efforts are needed to reduce formaldehyde emission, one of which is using activated charcoal. The use of bagasse-activated charcoal in the manufacture of particleboard mixture of bagasse and sawdust to minimize formaldehyde emission has been carried out. This research aims to evaluate the resistance of low-emission particleboard against three different wood-decay fungi species. Activated charcoal is made by carbonizing bagasse at 300 °C for 2.5 h followed by carbon activation using 0.1 M HCl solution for 24 h. The particleboard used in this study has to vary formaldehyde emission, such as 5.03, 3.95, 3.61, 3.38, 3.31, 3.28, 3.2, 3.19, 3.13, 3.05 mg/L. Particleboard was exposed to two white-rot wood-decay species white-rot Coriolus versicolor (COV) and Schizophyllum commune (SC), and brown-rot Tyromyces palustris (TYP), for three months testing period. The results showed that particleboard with low formaldehyde emission (high activated charcoal content) was more resistant to all wood-decay species.