Due to their intrinsic magnetic characteristics and multifunctional design, magnetic nanoparticles are highly functionalizable cancer therapeutic tools for diagnostics, monitoring, and treatment. The magnetic characteristics of magnetic nanoparticles (MNPs) have made them a study hotspot and biomedical staple in recent decades. Magnetofection is discussed in this minireview during every dynamic gene delivery phase (cellular uptake, gene binding intracellular trafficking,endosomal escape and in vivo targeting). HT treatment alone has had mixed clinical outcomes. Temperature and duration affect HT-induced cell death. The apoptotic route and degree of apoptosis induced by HT varies by cancer cell type. Apoptosis in numerous cell types is caused by HT-induced reactive oxygen species. However, signal transduction’s mechanism and genes remain unknown. In this study, we discuss the molecular mechanism of HT-induced apoptosis, Nanoparticle-based phototherapy, such as photothermal therapy (PTT), which creates heat, and photodynamic treatment (PDT), which generates ROS and stimulates anti-tumor immunity, aims to eliminate tumors. Its therapeutic effectiveness, little invasion, and insignificant side effects have garnered interest and investigation in recent years. In this article, PTT and PDT nanomaterial generations are addressed independently. Nano-particles can cure cancers, melanoma, rheumatoid, and other disorders depending on their combined route. This paper describes how pathological treatment induces cancer cell apoptosis by ROS formed by PDT, immunogenic cell death to mature dendritic cells, which activate CD4+, CD8+, and memory T cells, and inhibits, STAT3 heat shock protein (HSPs) signal pathway, and so on.