Freshwater ecosystems are continuously impacted by contaminants from urban runoffUrban runoffs, a process that becomes more pronounced during rainfall events. This runoff originates from various urban surfaces, including roads, pavements, residential, industrial, and green areas, carrying a diverse array of micropollutantsMicropollutants that pose a significant threat to surface waterWater quality and aquatic ecosystem healthHealth. ClimateClimate changeClimate change has further exacerbated these challenges by increasing the frequency and intensity of extreme weather events in urban areas. These conditions enhance the mobilization of pollutants through rainfall wash-off, leading to contamination levels that exceed normal conditions. Additionally, prolonged dry periods allow pollutants to accumulate on urban surfaces, which are then flushed into urban streams and waterWater bodies during subsequent rainfall, further impacting biodiversity and waterWater quality. To address these issues, we propose a comprehensive approach to assess the presence of micropollutantsMicropollutants in urban runoffUrban runoffs during extreme weather events. Our methodology employs liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMSHRMS), integrating both target and suspect screeningTarget and suspect screening methods to identify and quantify a broad range of micropollutantsMicropollutants. Understanding the composition of micropollutantsMicropollutants in urban runoffUrban runoffs is crucial for developing effective mitigation strategies. These findings will contribute to improved waterWater managementWater management and the protection of freshwater ecosystems in the face of evolving climateClimate conditions.