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Marker-free, quantitative in vitro imaging of living cell cultures with light microscopy is an important tool for various research areas in biosciences. Digital holographic microscopy (DHM) allows the characterization of cells and tissues and the quantification of cellular processes without staining or labeling. The method is fast and minimally invasive, biological samples can therefore be measured over extended time periods and downstream analyzed with further methods. The chapter first briefly introduces the analysis of living cells and tissue sections with DHM. Moreover, methods for extracting biophysical parameters from quantitative DHM phase contrast images are explained. Finally, selected applications illustrate the application in in vitro live cell analysis and in the quantification of physical tissue properties relevant for immunology.

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Quantitative Digital Holographic Phase Contrast Microscopy: Biophysical Characterization of Immunological Processes, Cells, and Tissues

  • 章节

  • 发表日期：

    2026年

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  • Björn Kemper,
  • Thomas Liedtke,
  • Jürgen Schnekenburger

摘要

Marker-free, quantitative in vitro imaging of living cell cultures with light microscopy is an important tool for various research areas in biosciences. Digital holographic microscopy (DHM) allows the characterization of cells and tissues and the quantification of cellular processes without staining or labeling. The method is fast and minimally invasive, biological samples can therefore be measured over extended time periods and downstream analyzed with further methods. The chapter first briefly introduces the analysis of living cells and tissue sections with DHM. Moreover, methods for extracting biophysical parameters from quantitative DHM phase contrast images are explained. Finally, selected applications illustrate the application in in vitro live cell analysis and in the quantification of physical tissue properties relevant for immunology.