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Two-photon microscopy (2 PM) is a modern imaging technology that enables the observation of dynamic cellular processes deep within the tissues and organs of living organisms. The use of a pulsed ultrafast infrared laser allows high light penetration into the tissue under investigation. As a result, this imaging technology is well suited for a wide range of in vivo applications. This chapter briefly introduces the biophysical basics of 2 PM and illustrates its current applications in intravital imaging.

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In Vivo Immunostaining and Intravital Two-Photon Microscopy

  • 章节

  • 发表日期：

    2026年

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  • Marco Gallus,
  • Maria Gabriele Bixel

摘要

Two-photon microscopy (2 PM) is a modern imaging technology that enables the observation of dynamic cellular processes deep within the tissues and organs of living organisms. The use of a pulsed ultrafast infrared laser allows high light penetration into the tissue under investigation. As a result, this imaging technology is well suited for a wide range of in vivo applications. This chapter briefly introduces the biophysical basics of 2 PM and illustrates its current applications in intravital imaging.