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The widespread use of immunoassays in routine and research laboratories is based on two important features: in many cases, assays can be carried out relatively quickly and easily; moreover, the target substance can be detected in a mixture of many different substances, present in a millionfold excess of the target substance. Both features are based on the detection molecules used in the immuno-assay, the antibodies. Antibodies “recognize” the substance to be analyzed, the analyte, within a heterogeneous group of molecules. This chapter is an introduction to the methodology and diversity of immunoassays and prepares the reader for further chapters of this textbook.

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Introduction to Immunoassays

  • 章节

  • 发表日期：

    2026年

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  • Arnold Maria Raem,
  • Claudia Goldman,
  • Fabio Rizzo,
  • Uwe Schedler,
  • Fridtjof Lechhart,
  • Enrico Pelz,
  • Bruno Rosen,
  • Mazen Juratli

摘要

The widespread use of immunoassays in routine and research laboratories is based on two important features: in many cases, assays can be carried out relatively quickly and easily; moreover, the target substance can be detected in a mixture of many different substances, present in a millionfold excess of the target substance. Both features are based on the detection molecules used in the immuno-assay, the antibodies. Antibodies “recognize” the substance to be analyzed, the analyte, within a heterogeneous group of molecules. This chapter is an introduction to the methodology and diversity of immunoassays and prepares the reader for further chapters of this textbook.