Additive manufacturing has revolutionized product development, enabling the creation of innovative fasteners with enhanced functionality and design flexibility. The objective of this research is to explore the development of fasteners where one part is fabricated with polylactic acid (PLA) and the other with thermoplastic polyurethane (TPU), both deposited on poly methyl methacrylate (PMMA) substrates by material extrusion. Different dimensions of the fasteners are investigated to optimize performance. The study uses cyclic testing to evaluate the opening and closing behaviour, providing insight into the long-term durability of the elements. In addition, stress simulations are performed to understand mechanical properties and failure points. The results show significant variations in performance based on penetration depth and dimension of the elements, providing valuable data for the future application of additive manufacturing in product development. The results highlight the potential for customized, durable fasteners in various industrial applications.