Polysaccharide biofilms 3D-printed have provided a significant level of interest because of their sustainable, biodegradable, and changeable properties. The biofilms have high adsorption capacity, mechanical strength, and controllable porosity, making them useful in the elimination of pollutants like heavy metals, organic, and microplastic substances in aqueous and soil matrices. In this chapter, the synthesis, characterization, and use of 3D-printed polysaccharide biofilms are outlined with a stress on the role of these biofilms in sustainable environmental practices. Major developments, including the strategies of functionalization, scalability, and field deployment, are discussed. In addition, barriers in cost, reproducibility, and regulatory compliance are examined, as well as the potential opportunities to increase their environmental impact.