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In recent years, advancements in 3D printing and additive manufacturing have revolutionized biomedical research, enabling the creation of customizable, low-cost devices for a wide range of applications. This study introduces a DIY, 3D-printed smart device designed for real-time monitoring of lactate and glucose during cell culture growth. Utilizing Internet of Things (IoT) technology, the device is compatible with standard six-well plates, a common laboratory tool for cell culture and other biological experiments. These plates feature six separate wells arranged in two rows of three, allowing a parallel format, and the device integrates in situ biosensors for continuous measurement of cellular metabolites. Fabricated from PETG using fused filament fabrication (FFF), the device is durable, capable of withstanding sterilization, and supports orbital shaking to ensure uniform nutrient distribution across cell cultures. Data is wirelessly transmitted to a web platform and mobile application, allowing for intuitive visualization and remote monitoring of metabolic trends. Proof-of-concept experiments demonstrated accurate metabolite measurements, showcasing the device’s potential for tracking cellular metabolism in real-time. This approach emphasizes the power of 3D-printed, IoT-enabled biosensors to revolutionize laboratory research, offering accessible, customizable tools for cell culture studies, organoid development, and pharmaceutical testing. Through a DIY approach, this device empowers researchers to build intelligent, cost-effective solutions that enhance the reproducibility and scalability of metabolic analysis in organoid cultures.

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Smart Device for Real-Time Monitoring of Biometabolites During Cell Culture Growth

  • 会议论文

  • 发表日期：

    2026年

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  • Sotirios Pemas,
  • Alexandros Kanlis,
  • Iason Malkotsis,
  • Christina Nioti,
  • Christina Sarmazanidou,
  • Dimosthenis Ioannidis,
  • Eleftheria Maria Pechlivani

摘要

In recent years, advancements in 3D printing and additive manufacturing have revolutionized biomedical research, enabling the creation of customizable, low-cost devices for a wide range of applications. This study introduces a DIY, 3D-printed smart device designed for real-time monitoring of lactate and glucose during cell culture growth. Utilizing Internet of Things (IoT) technology, the device is compatible with standard six-well plates, a common laboratory tool for cell culture and other biological experiments. These plates feature six separate wells arranged in two rows of three, allowing a parallel format, and the device integrates in situ biosensors for continuous measurement of cellular metabolites. Fabricated from PETG using fused filament fabrication (FFF), the device is durable, capable of withstanding sterilization, and supports orbital shaking to ensure uniform nutrient distribution across cell cultures. Data is wirelessly transmitted to a web platform and mobile application, allowing for intuitive visualization and remote monitoring of metabolic trends. Proof-of-concept experiments demonstrated accurate metabolite measurements, showcasing the device’s potential for tracking cellular metabolism in real-time. This approach emphasizes the power of 3D-printed, IoT-enabled biosensors to revolutionize laboratory research, offering accessible, customizable tools for cell culture studies, organoid development, and pharmaceutical testing. Through a DIY approach, this device empowers researchers to build intelligent, cost-effective solutions that enhance the reproducibility and scalability of metabolic analysis in organoid cultures.