Nanomaterials (NMs) offer several advantages due to their fascinating properties and are being widely used in agriculture. This involves their widespread applications in various fields, including biomedicine, engineering, food, cosmetics, sensing, and energy. Nanotechnology offers unique opportunities for plant scientists and agricultural biotechnologists to develop and implement innovative tools and procedures for enhancing commercially valuable plants and crops. More specifically, the implementation of NMs within plant tissue culture and cell suspension systems has shown substantial potential to stimulate cell growth, increase nutrient absorption, and enhance the secondary metabolites yield. These applications contribute to the efficiency of in vitro plant culture and offer prospects for mass production of biologically derived active compounds for pharmaceutical or industrial applications. The current chapter aims to gather the latest advancements in nanotechnology and cell suspension culture, focusing on the favourable and negative impacts of the nanoparticles (NPs). The toxicity and safety issues associated with exposing plants and their surrounding environment to NPs have been outlined. Advancing plant nanobiotechnology necessitates further research to resolve unanswered questions and clarify underlying mechanisms, which is essential for achieving significant scientific progress in the agriculture field.