The increasing utilization of nanotechnology has resulted in the release of nanoparticles (NPs) into the environment, prompting worries about their effects on plant health and ecosystem stability. Plants are primary producers, and are particularly vulnerable to nano pollution, which can interfere with basic and secondary metabolic pathways that are essential for defense, development, and adaptation. Metabolomics, a comprehensive technique for characterizing small molecules, is a great technique for investigating the metabolic impacts of NPs on plants. Researchers can identify metabolic alterations at high resolution using methods including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy. This chapter investigates the metabolomic changes caused by NP exposure, emphasizing changes in basic metabolism (photosynthesis, energy production, and nutritional assimilation) and secondary metabolism, which includes defense-related chemicals such as phenolics, flavonoids, and terpenoids. Metabolomics analyzes these metabolite profiles to determine how plants react to oxidative stress, interruption of nutrient absorption, and interference with cellular metabolic pathways brought on by nanopollution. Researchers may build a comprehensive understanding of plant metabolic adaptability to NPs exposure by relating metabolomics data with results of other omics studies. This allows them to make informed decisions about environmental safety and sustainable agriculture practices. This chapter emphasizes the importance of metabolomics in knowing more about plant interactions with NPs and identifies future research options for minimizing the impacts of nano-pollution on plant systems.