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Microbial denitrification technology is one of the effective methods for nitrogen purification in water. Most wastewater has physical and chemical properties such as high pH, high salinity, and high organic matter, which can easily affect microbial activity and metabolic ability. In order to screen for microbial strains with specific abilities, this study isolated and screened a strain of alkali tolerant heterotrophic nitrifying aerobic denitrifying bacteria (HN-AD) J7-2 from sediments in residential areas of the Baiyangdian Basin. The strain was identified as Bacillus altitudinis by 16S rRNA sequencing and can grow rapidly in the pH range of 8–9. Under different nitrogen source conditions, the nitrogen concentration in water can be significantly reduced. The 24-h removal rates of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen were 98.43%, 71.45%, and 81.08%, respectively. The utilization efficiency of these three nitrogen sources was in the order of ammonia nitrogen > nitrite nitrogen > nitrate nitrogen. It was also found that the utilization efficiency of ammonia nitrogen was significantly enhanced in the composite nitrogen source components. The research results indicate that the screened heterotrophic nitrification aerobic denitrification (HN-AD) strain DC3-2 can be used as a candidate strain for biological denitrification agents and denitrification mechanism research, and can be applied to the ecological restoration of water quality in rural ponds and aquaculture effluent.

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Isolation, Identification, and Denitrification Ability of Alkaline Tolerant Heterotrophic Nitrification Aerobic Denitrification Strain DC3-2 in the Sediment of Baiyangdian Lake

  • 会议论文

  • 发表日期：

    2026年

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  • Liyuan Wang,
  • He Zhou,
  • Meng Qin,
  • Chunmao Liu,
  • Miwei Shi,
  • Zhenhua Jia,
  • Chunying Li,
  • Fangtong Wu,
  • Shaopeng Ma

摘要

Microbial denitrification technology is one of the effective methods for nitrogen purification in water. Most wastewater has physical and chemical properties such as high pH, high salinity, and high organic matter, which can easily affect microbial activity and metabolic ability. In order to screen for microbial strains with specific abilities, this study isolated and screened a strain of alkali tolerant heterotrophic nitrifying aerobic denitrifying bacteria (HN-AD) J7-2 from sediments in residential areas of the Baiyangdian Basin. The strain was identified as Bacillus altitudinis by 16S rRNA sequencing and can grow rapidly in the pH range of 8–9. Under different nitrogen source conditions, the nitrogen concentration in water can be significantly reduced. The 24-h removal rates of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen were 98.43%, 71.45%, and 81.08%, respectively. The utilization efficiency of these three nitrogen sources was in the order of ammonia nitrogen > nitrite nitrogen > nitrate nitrogen. It was also found that the utilization efficiency of ammonia nitrogen was significantly enhanced in the composite nitrogen source components. The research results indicate that the screened heterotrophic nitrification aerobic denitrification (HN-AD) strain DC3-2 can be used as a candidate strain for biological denitrification agents and denitrification mechanism research, and can be applied to the ecological restoration of water quality in rural ponds and aquaculture effluent.