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Global shrimp processing generates ~30 million tons of shell waste annually, causing severe environmental pollution while wasting valuable resources (30–40% protein, 20–30% chitin, 30–50% CaCO₃). To address this, a novel Lactococcus lactis strain XQYZ was isolated from Chaoyang fishpond sediments using CaCO₃-free MRS screening medium. During 6-day fermentation (30 g/L shrimp shell powder, 37 °C, 180 rpm), XQYZ achieved 91.49% ± 0.50% demineralization efficiency (DM%)—significantly outperforming comparative strains (SCX: 90.37%; ZZZY: 83.71%). The strain exhibited exceptional acidification capacity (pH 4.1–4.2 at 48 h) and robust growth kinetics with exponential phase at 5–20 h (OD₆₀₀ max = 1.8). Mechanistic studies revealed that organic acids (e.g., lactate) solubilized calcium complexes, enabling >90% mineral removal. Phylogenetic and morphological analysis confirmed its identity as gram-positive cocci with smooth colonies. This bio-based approach provides an eco-friendly alternative to chemical demineralization, facilitating sustainable chitin recovery for aquaculture waste valorization and bioplastic production.

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High-Efficiency Bio-Demineralization of Shrimp Shells Using a Novel Lactococcus Lactis Isolate

  • 会议论文

  • 发表日期：

    2026年

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  • Yunfeng Liu,
  • Shuai Liang,
  • Jianwei Wang,
  • Kaihua Geng,
  • Weiyu Du,
  • Shangwen Liu,
  • Lianshun Wang,
  • Yuting Cong,
  • Yanan Lu,
  • Guojun Yang

摘要

Global shrimp processing generates ~30 million tons of shell waste annually, causing severe environmental pollution while wasting valuable resources (30–40% protein, 20–30% chitin, 30–50% CaCO₃). To address this, a novel Lactococcus lactis strain XQYZ was isolated from Chaoyang fishpond sediments using CaCO₃-free MRS screening medium. During 6-day fermentation (30 g/L shrimp shell powder, 37 °C, 180 rpm), XQYZ achieved 91.49% ± 0.50% demineralization efficiency (DM%)—significantly outperforming comparative strains (SCX: 90.37%; ZZZY: 83.71%). The strain exhibited exceptional acidification capacity (pH 4.1–4.2 at 48 h) and robust growth kinetics with exponential phase at 5–20 h (OD₆₀₀ max = 1.8). Mechanistic studies revealed that organic acids (e.g., lactate) solubilized calcium complexes, enabling >90% mineral removal. Phylogenetic and morphological analysis confirmed its identity as gram-positive cocci with smooth colonies. This bio-based approach provides an eco-friendly alternative to chemical demineralization, facilitating sustainable chitin recovery for aquaculture waste valorization and bioplastic production.