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This study investigated optimal sterilization and reproduction methods to enhance laboratory-scale cultivation of Ulva spp., a green macroalga with significant applications in aquaculture and potential use for biofortification assays. Various sterilization protocols were tested, including Triton X-100, Ethanol and NaOCl, to evaluate their effectiveness in removing contaminants while preserving the integrity of Ulva spp. samples. Results showed that 0.5% Triton X-100 effectively eliminated contamination while maintaining chlorophyll, making it ideal for preserving wild-collected Ulva spp. For reproductive purposes, disinfection with 1% NaOCl for 30 s, despite its aggressive effects, ensured contamination-free samples and promoted swarmer release. Reproductive trials identified that chilled f/2 medium at 4 °C for 10 min as the optimal condition for swarmer production, yielding the highest numbers compared to other tested treatments. Observations of swarmer development into juvenile stages further validated the effectiveness of the optimized protocol. These findings provide a robust foundation for improving Ulva spp. cultivation techniques, with implications for aquaculture and bioresource applications.

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Optimizing Ulva Spp. Sterilization and Reproduction Techniques for Controlled Biofortification Assays in Laboratory Conditions

  • 会议论文

  • 发表日期：

    2026年

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  • Cláudia Lopes,
  • Salla Leppäkoski,
  • Marika Tossavainen,
  • Maria Manuel Gil,
  • Filipa R. Pinto,
  • Verónica Felício

摘要

This study investigated optimal sterilization and reproduction methods to enhance laboratory-scale cultivation of Ulva spp., a green macroalga with significant applications in aquaculture and potential use for biofortification assays. Various sterilization protocols were tested, including Triton X-100, Ethanol and NaOCl, to evaluate their effectiveness in removing contaminants while preserving the integrity of Ulva spp. samples. Results showed that 0.5% Triton X-100 effectively eliminated contamination while maintaining chlorophyll, making it ideal for preserving wild-collected Ulva spp. For reproductive purposes, disinfection with 1% NaOCl for 30 s, despite its aggressive effects, ensured contamination-free samples and promoted swarmer release. Reproductive trials identified that chilled f/2 medium at 4 °C for 10 min as the optimal condition for swarmer production, yielding the highest numbers compared to other tested treatments. Observations of swarmer development into juvenile stages further validated the effectiveness of the optimized protocol. These findings provide a robust foundation for improving Ulva spp. cultivation techniques, with implications for aquaculture and bioresource applications.