This study investigates the environmental and logistical implications of adopting Reusable Plastic Containers (RPCs) in the distribution of fresh fruits and vegetables. A combined approach based on Life Cycle Assessment (LCA) and digital twin modeling was applied to simulate different crate configurations and assess their performance. The analysis relies on primary data collected through on-field test campaigns, involving multiple product categories and crate formats with varying capacities. By comparing different operating scenarios, the study quantifies both environmental benefits and logistical savings, particularly in terms of unit load and vehicle utilization. The analysis includes a case study involving several major Italian retailers and a pooling service provider. Results obtained from the case study show measurable improvements, highlighting the potential of crate design choices to reduce environmental impacts while increasing handling efficiency. The methodology proves effective in supporting evidence-based decisions for more sustainable and efficient fresh food supply chains operations.