Dynamic preservation strategies are a promising approach to enhancing graft quality and extending preservation time for logistical and therapeutic purposes. Unlike normothermic machine perfusion, which aims to mimic physiologic conditions in the human body, hypothermic oxygenated perfusion operates under non-physiological conditions, eliciting a distinct mitochondrial response that contributes to its protective effect. Both ex-situ perfusion techniques can be utilized for viability assessment, a key feature paving the way for increased liver utilization to unlock the enormous donor pool in the future.